Keyword
iPSC, ESC, adipocyte, chondrocyte, transcription factor, epigenetics, chromatin
研究テーマ
- Gene Regulation
- Stem Cell Biology
研究ハイライト
Major Scientific Interests of the Group
Our group studies the regulation of eukaryotic gene expression, focusing on how transcription regulates cell differentiation. In particular, we are studying the roles of transcription factors and epigenetic changes in regulating iPSC generation, iPSC differentiation, as well as differentiation of adipocytes and chodrocytes.
Projects for Regular Students in Doctoral or Master’s Programs
1) Mechanistic analyses of the functional roles for Oct4, Sox2, Klf4 and c-myc during iPSC generation.
2) Analyses of epigenetic changes during iPSC generation.
3) Functional analyses of regulatory factors involved in adipocyte and chondrocyte differentiation.
4) In vivo imaging and mechanistic analyses of beige adipocyte differentiation in mouse
Study Programs for Short Stay Students (one week ~ one trimester)
1) Analysis of transcriptional regulation during adipocyte differentiation.
2) Induction of iPSCs using a Sendai virus-based vector.
研究の応用・展望
- We have established in vitro systems for generating iPSCs and for differentiating iPSCs into neural stem cells. Moreover, we also developed systems to generate adipocytes and chondrocytes from mesenchymal stem cells and iPSCs. Our systems are applicable for screening and testing chemical compounds for potential drug development.
文献・知財・作品
- Bui PL, Nishimura K, Seminario Mondejar G, Kumar A, Aizawa S, Murano K, Nagata K, Hayashi Y, Fukuda A, Onuma Y, Ito Y, Nakanishi M, Hisatake K. Template Activating Factor-Iα Regulates Retroviral Silencing during Reprogramming. Cell Rep. 29(7): 1909-1922 (2019).
- Fukuda A, Honda S, Fujioka N, Sekiguchi Y, Mizuno S, Miwa Y, Sugiyama F, Hayashi Y, Nishimura K, Hisatake K. Non-invasive in vivo imaging of UCP1 expression in live mice via near-infrared fluorescent protein iRFP720. PLoS One 14(11): e0225213 (2019).
- Nishimura K, Aizawa S, Nugroho FL, Shiomitsu E, Tran YTH, Bui PL, Borisova E, Sakuragi Y, Takada H, Kurisaki A, Hayashi Y, Fukuda A, Nakanishi M, Hisatake K: A role for KLF4 in promoting the metabolic shift via TCL1 during induced pluripotent stem cell generation. Stem Cell Reports 8(3), 787-801 (2017).
http://www.md.tsukuba.ac.jp/basic-med/biochem/gene/
70271236
医学医療系
Laboratory of Gene Regulation, Faculty of Medicine,
Other Faculty Members
Associate Professor: Aya Fukuda
Associate Professor: Ken Nishimura