Development of bioartificial organs from viewpoints of biomedical engineering and tissue engineering. | 三好浩稔 | 筑波大学研究者カタログ

代表者 : 三好 浩稔  

Keyword

Hematopoietic stem cells, ex vivo expansion, 3D culture, bioartificial organs

研究テーマ

  • Development of bioartificial organs from viewpoints of biomedical engineering and tissue engineering.

研究ハイライト

Major Scientific Interests of the Group

1) Development of bioartificial organs by using tissue engineering approach.

2) Establishment of novel 3Dculture methods mimicking in vivo microenvironment.

Projects for Regular Students in Doctoral or Master’s Programs

1) Development of ex vivo expansion system of hematopoietic stem cells

2) Development of bioartificial livers

3) Establishment of novel bioreactor systems applicable to bioartificial organs

Study Programs for Short Stay Students (one week – one trimester)

1)3D culture techniques including preparation of 3D scaffolds, cell seeding into the scaffolds,　cryopreservation of 3D culture cells, and assays of the cells.

研究の応用・展望

  • The goal of our present research is establishing an efficient and practical ex vivo expansion system of hematopoietic stem cells derived from umbilical cord blood. This system is expected to contribute wider application of umbilical cord blood transplantation.

文献・知財・作品

  • Miyoshi H, Abo K, Hosoya D, Matsuo K, Utsumi Y. Effects of mouse fetal liver cell culture density on hematopoietic cell expansion in three-dimensional cocultures with stromal cells. Int J Artif Organs, 2021. DOI: 10.1177/0391398821996377
  • Miyoshi H, Iwamoto A, Koyama T. Growth and albumin secretion oif mouse fetal liver cellscryopreserved within porous polymer scaffolds as a viable cell source for bioartificial livers. J Biosci Bioeng, 130: 212-216, 2020.
  • Miyoshi H, Shimizu Y, Yasui Y, Sugiyama S. Expansion of mouse primitive hematopoietic cells in three-dimensional cultures on chemically fixed stromal cell layers. Cytotechnology, 72: 741-750, 2020.
  • Miyoshi H, Morita M, Ohshima N, Sato C. Expansion of mouse hematopoietic progenitor cells in three-dimensional cocultures on frozen-thawed stromal cell layers formed within porous scaffolds. Exp Hematol, 43: 115-124, 2015.

http://www.md.tsukuba.ac.jp/bm-engng/

70292547

医学医療系

Department of Biomedical Engineering, Faculty of Medicine