● Genetically modified mouse ● Kidney disease ● Diabetes

Genetically modified mice, genome editing, kidney disease, diabetes

●Rapid production of genetically modified mice and human disease model mice by genome editing using CRISPR/Cas9.
●Development of glomerular disease prevention and onset suppression methods by enhancing renal podocyte function.
●Maintaining pancreatic β-cell function and promoting regeneration method of pancreatic β-cell.
Anatomy and Embryology, Laboratory Animal Resource Center in Transborder Medical Research Center
satoruta@md.tsukuba.ac.jp
http://www.md.tsukuba.ac.jp/basic-med/anatomy/embryology/index.html
50271896 https://orcid.org/0000-0002-8540-7760
Department of Biomedical Science, Faculty of Medicine
Satoru Takahashi
Genetically modified mice, genome editing, kidney disease, diabetes
●Mizuno S, et al. Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the
CRISPR/Cas9 system. Mamm Genome. 2014 Aug;25(7-8):327-34.
●Tran MTN, et al. MafB is a critical regulator of complement component C1q. Nat Commun. 2017 Nov 22;8(1):1700.
●Sato Y, et al. A mutation in transcription factor MAFB causes Focal Segmental Glomerulosclerosis with Duane Retraction
Syndrome. Kidney Int. 2018 Aug;94(2):396-407.
●Jung Y, et al. Isl1β Overexpression With Key β Cell Transcription Factors Enhances Glucose-Responsive Hepatic Insulin
Production and Secretion. Endocrinology. 2018 Feb 1;159(2):869-882.
December.2020
https://ura.sec.tsukuba.ac.jp/unit-members?kid=50271896
● Genetically modified mouse ● Kidney disease ● Diabetes
Genome editing using CRISPR/Cas9 can produce
genetically modified mice using fertilized mouse
eggs. Not only knockout, knockin, and conditional
knockout mice, human disease models can be
rapidly created by introducing mutations identified in
human cases into mice. These mouse models are
useful to identify molecular mechanisms and develop
treatment methods. In addition, we are analyzing
functions of Large MAF transcription factors using
genetically modified mice, which can be applied to
the study of kidney disease and diabetes.
Collaborators
Associate Professor; Takashi Kudo, Eiji Warabi
Assistant Professor; Michito Hamada, Akihiro Kuno